Multiple effects of silymarin on the hepatitis C virus lifecycle.

Silymarin, an extract from milk thistle (Silybum marianum), and its purified flavonolignans have been recently shown to inhibit hepatitis C virus (HCV) infection, both in vitro and in vivo. In the current study, we further characterized silymarin's antiviral actions. Silymarin had antiviral effects against hepatitis C virus cell culture (HCVcc) infection that included inhibition of virus entry, RNA and protein expression, and infectious virus production. Silymarin did not block HCVcc binding to cells but inhibited the entry of several viral pseudoparticles (pp), and fusion of HCVpp with liposomes. Silymarin but not silibinin inhibited genotype 2a NS5B RNA-dependent RNA polymerase (RdRp)

Grape seed proanthocyanidin extract ameliorates monosodium iodoacetate-induced osteoarthritis

Source Department of Internal Medicine, Bucheon St. Mary's Hospital, Korea. Abstract Osteoarthritis (OA) is an age-related joint disease that is characterized by degeneration of articular cartilage and chronic pain. Oxidative stress is considered one of the pathophysiological factors in the progression of OA. We investigated the effects of grape seed proanthocyanidin extract (GSPE), which is an antioxidant, on monosodium iodoacetate (MIA)-induced arthritis of the knee joint of rat, which is an animal model of human OA. GSPE (100 mg/kg or 300 mg/kg) or saline was given orally three times per week for 4 weeks after the MIA injection. Pain was

Not in the mind of neurasthenic lazybones but in the cell nucleus: patients with chronic fatigue syndrome have increased production of nuclear factor kappa beta

MCare4U Outpatient Clinics, Olmenlaan 9, 2610 Wilrijk, Belgium. There is now some evidence that chronic fatigue syndrome is accompanied by an activation of the inflammatory response system and by increased oxidative and nitrosative stress. Nuclear factor kappa beta (NFkappabeta) is the major upstream, intracellular mechanism which regulates inflammatory and oxidative stress mediators. In order to examine the role of NFkappabeta in the pathophysiology of CFS, this study examines the production of NFkappabeta p50 in unstimulated, 10 ng/mL TNF-alpha (tumor necrosis factor alpha) and 50 ng/mL PMA (phorbolmyristate acetate) stimulated peripheral blood lymphocytes of 18 unmedicated patients with CFS and 18

Curcumin synergistically potentiates the growth inhibitory and pro-apoptotic effects of celecoxib in pancreatic adenocarcinoma cells

Department of Cancer Prevention, Tel Aviv Medical Center, Israel. BACKGROUND AND AIM: Adenocarcinoma of the Pancreas is a leading cause of cancer-related mortality, accounting for an estimated 30,000 deaths per year in the United States. Multiple studies have indicated that specific cyclooxygenase-2 (COX-2) inhibitors may serve in the prevention and treatment of a variety of malignancies including pancreatic adenocarcinoma. Recent studies had shown that the long-term use of high concentration of COX-2 inhibitors is not toxic free and may be limited due to serious gastrointestinal and cardiovascular side effects. The chemopreventive efficacy of the phytochemical, curcumin has been demonstrated in

Curcumin: the Indian solid gold

Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA. Turmeric, derived from the plant Curcuma longa, is a gold-colored spice commonly used in the Indian subcontinent, not only for health care but also for the preservation of food and as a yellow dye for textiles. Curcumin, which gives the yellow color to turmeric, was first isolated almost two centuries ago, and its structure as diferuloylmethane was determined in 1910. Since the time of Ayurveda (1900 Bc) numerous therapeutic activities have been assigned to turmeric for a wide variety of diseases and conditions, including

Glycyrrhizin as antiviral agent against Hepatitis C Virus

Copyright ©2011 Ashfaq et al; licensee BioMed Central Ltd. Background Hepatitis C virus is a major cause of chronic liver diseases which can lead to permanent liver damage, hepatocellular carcinoma and death. The presently available treatment with interferon plus ribavirin, has limited benefits due to adverse side effects such as anemia, depression, fatigue, and "flu-like" symptoms. Herbal plants have been used for centuries against different diseases including viral diseases and have become a major source of new compounds to treat bacterial and viral diseases. Material The present study was design to study the antiviral effect of Glycyrrhizin (GL) against HCV.

Glycine propionyl-L-carnitine produces enhanced anaerobic work capacity with reduced lactate accumulation in resistance trained males.

From this website. BACKGROUND: Recent research has indicated that short term administration of glycine propionyl-L-carnitine (GPLC) significantly elevates levels of nitric oxide metabolites at rest and in response to reactive hyperaemia. However, no scientific evidence exists that suggests such supplementation enhances exercise performance in healthy, trained individuals. The purpose of this study was to examine the effects of GPLC on the performance of repeated high intensity stationary cycle sprints with limited recovery periods in resistance trained male subjects. METHODS: In a double-blind, placebo-controlled, cross-over design, twenty-four male resistance trained subjects (25.2 +/- 3.6 years) participated in two test sessions separated

Glycine Propionyl-L-Carnitine HCL, USP (GlycoCarn®) for Human Health and Performance

Richard J. Bloomer, PhD Department of Health and Sports Sciences The University of Memphis Dietary supplementation with the naturally occurring nutrient L-carnitine has been extensively studied as an aid to improve fatty acid metabolism and aerobic exercise capacity, to provide antioxidant benefits, and to enhance blood flow to active tissues (e.g., skeletal muscle and heart). While multiple forms of carnitine have been studied and are currently available, Propionyl-L-Carnitine (PLC) has been shown to best support healthy heart and skeletal muscle function. A patented and safe USP dietary form of PLC has been developed which is molecularly bonded to glycine, one

Progesterone – The Feel Good Hormone – Part 1

By Ward Dean, M.D. This is the first of a two-part series about common health problems of women, and how, in clinical trials, natural progesterone supplementation can be of great benefit. In the second installment, appearing in next months Nutritional News, Dr. Dean discusses progesterone supplementation and osteoporosis. Some of the most common symptoms physicians hear from their female patients are problems with weight gain, fatigue, loss of libido, depression, headaches, joint pain and mood swings. Other frequently discovered problems include uterine fibroids, cancer, fibrocystic breast disease, menstrual problems, autoimmune disorders, pre-menopausal bone loss and a high incidence of osteoporosis

Progesterone – The Feel Good Hormone – Part 2

By Ward Dean, M.D. Some of the most common symptoms physicians hear from their female patients are problems with weight gain, fatigue, loss of libido, depression, headaches, joint pain and mood swings. Other frequently discovered problems include uterine fibroids, cancer, fibrocystic breast disease, menstrual problems, autoimmune disorders, pre-menopausal bone loss and a high incidence of osteoporosis after menopause. Many physicians and scientists are becoming increasingly aware of a common link between these symptoms and diseases and that common link is often an imbalance between the primary female sex hormones, progesterone and estrogen.(1) Studies show that by restoring the estrogen: progesterone

Vascular basis of Alzheimer’s pathogenesis

Article taken from this website. Source Division of Neuropathology, University of California-San Diego, 1363 Shinly, Suite 100, Escondido, CA 92026, USA. Abstract Considerable evidence now indicates that Alzheimer's disease (AD) is primarily a vascular disorder. This conclusion is supported by the following evidence: (1) epidemiologic studies linking vascular risk factors to cerebrovascular pathology that can set in motion metabolic, neurodegenerative, and cognitive changes in Alzheimer brains; (2) evidence that AD and vascular dementia (VaD) share many similar risk factors; (3) evidence that pharmacotherapy that improves cerebrovascular insufficiency also improves AD symptoms; (4) evidence that preclinical detection of potential AD

What Really Causes Heart Disease?

The following article is taken from this website. ____________ We physicians with all our training, knowledge and authority often acquire a rather large ego that tends to make it difficult to admit we are wrong. So, here it is. I freely admit to being wrong. As a heart surgeon with 25 years experience, having performed over 5,000 open-heart surgeries, today is my day to right the wrong with medical and scientific fact. I trained for many years with other prominent physicians labelled "opinion makers." Bombarded with scientific literature, continually attending education seminars, we opinion makers insisted heart disease resulted from

Saliva vs Serum

Saliva testing has been used to measure hormones since the late 1960s and has many advantages over serum testing. The most significant feature of saliva testing is that it reflects the non-protein bound 'free' fraction of hormones at a given point of time.1 As steroid hormones are predominantly bound to carrier proteins (cortisol binding protein, sex hormone binding globulin and albumin) in the blood, the unbound fraction is considered more readily available to the cells of the body (see Diagram 1). It is these 'free' hormones that best reflect a patient’s hormonally-related symptoms, rather than total or bound hormone levels

Nitric oxide prevents cardiovascular disease and determines survival in polyglobulic mice overexpressing erythropoietin

Nitric oxide (NO) induces vasodilatatory, antiaggregatory, and antiproliferative effects in vitro. To delineate potential beneficial effects of NO in preventing vascular disease in vivo, we generated transgenic mice overexpressing human erythropoietin. These animals induce polyglobulia known to be associated with a high incidence of vascular disease. Despite hematocrit levels of 80%, adult transgenic mice did not develop hypertension or thromboembolism. Endothelial NO synthase levels, NO-mediated endothelium-dependent relaxation and circulating and vascular tissue NO levels were markedly increased. Administration of the NO synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) led to vasoconstriction of peripheral resistance vessels, hypertension, and death of transgenic mice,